Journal: International Journal of Molecular Sciences

Article Title: The Microarchitecture of Pancreatic Cancer as Measured by Diffusion-Weighted Magnetic Resonance Imaging Is Altered by T Cells with a Tumor Promoting Th17 Phenotype

doi: 10.3390/ijms21010346

Figure Lengend Snippet: Receptors for IL21 and IL26 in PDAC. ( A ) Immunohistochemistry (IHC) of PDAC samples with no expression and high expression of IL10RB, IL20RA, or IL21R, in comparison to non-neoplastic pancreatic parenchyma. Arrow head: tumor cells ( B ) Double staining for IL10RB (red) and IL20RA (brown) shows co-localization (arrow, red-brown cells). ( C ) Bubble charts show the dependencies of the Allred score for IL20RA from Allred IL10RB (left), Allred IL21R from Allred IL10RB (middle), and Allred IL21R from Allred IL20RA (right). The diameter of the bubbles indicates the frequency of occurrence.

Article Snippet: After 24 h, cells were fixed with 2 % PFA and incubated with a rabbit anti-human IL10RB antibody (dilution: 1:50; NBP2–33492, NovusBiologicals) and a mouse anti-human IL20RA antibody (dilution: 1:50; NBP2–11695, Novus Biologicals), at 4 °C overnight.

Techniques: Immunohistochemistry, Expressing, Comparison, Double Staining

Journal: International Journal of Molecular Sciences

Article Title: The Microarchitecture of Pancreatic Cancer as Measured by Diffusion-Weighted Magnetic Resonance Imaging Is Altered by T Cells with a Tumor Promoting Th17 Phenotype

doi: 10.3390/ijms21010346

Figure Lengend Snippet: PDAC cell lines. ( A ) Laser scan microscopy and Western blotting of the PDAC cell lines AsPC-1, BxPC-3, and Panc-1 incubated with anti-IL10RB and anti-IL20RA antibodies shows expression and colocalization of both receptors in all three cell lines. ( B ) Western blotting of PDAC cells shows IL26 induced phosphorylation of STAT3 (left panel) and ERK1/2 (right panel) in a time-dependent manner.

Article Snippet: After 24 h, cells were fixed with 2 % PFA and incubated with a rabbit anti-human IL10RB antibody (dilution: 1:50; NBP2–33492, NovusBiologicals) and a mouse anti-human IL20RA antibody (dilution: 1:50; NBP2–11695, Novus Biologicals), at 4 °C overnight.

Techniques: Microscopy, Western Blot, Incubation, Expressing, Phospho-proteomics